Research and Innovation


CFTN Patents Issued:

Ratiometric Surface Plasmon Coupled Emission Detector, US Patent # 8,159,676, 04/17/2012, (Z. Gryczynski, I. Gryczynski, E. Matveeva, J. Borejdo).

Surface Plasmon Assisted Microscopy, US Patent # 7,956,989, 06/07/2011, (Z. Gryczynski, I. Gryczynski, J. Borejdo, N. Calander).

Peer-Reviewed Publications

Sabnis S, Sabnis N, Raut S, Lacko AG (2015) Magnetically Guided Drug Delivery via Reconstituted High Density Lipoprotein (rHDL) Nanoparticles. SAJ Nanosci Nanotech 1(1):

Kokate RA, Thamake SI, Chaudhary P, Mott B, Raut S, Vishwanatha JK, Jones HP. Enhancement of anti-tumor effect of particulate vaccine delivery system by ‘Bacteriomimetic’ CpG functionalization of poly-lactic-co-glycolic acid nanoparticles. Nanomedicine, Nanomedicine (Lond). 2015. 10(6), 915-29

Kimball JD, Raut S, Jameson LP, Smith NW, Gryczynski Z, Dzyuba SV. BODIPY–BODIPY dyad: assessing the potential as a viscometer for molecular and ionic liquids. RSC Adv., 2015,5, 19508-19511

Chib R, Raut S, Shah S, Grobelna B, Rich R, Sorensen TJ, Laursen BW, Grajek H, Gryczynski Z, Gryczynski I. Steady state and time-resolved fluorescence studies of azadioxatriangulenium (ADOTA) fluopophore in silica and PVA thin films. (2015) Dyes and Pigments 117, 16-23.

Shtoyko T, Raut S, Rich R, Sronce RJ, Fudala R, Mason RN, Akopova I, Gryczynski Z, Gryczynski I.Preparation of Plasmonic Platforms of Silver Wires on Gold Mirrors and their Applications to Surface Enhanced Fluorescence. (2014) Applied Materials and Interfaces 6, 18780-18787.

Raut S, Kimball J, Fudala R, Doan H, Maliwal B, Sabnis N, Lacko A, Gryczynski I, Zyuba S, Gryczynski Z. A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicing and cellular environments. (2014) Phys.Chem.Chem.Phys. 16, 27037-27042.

Borejdo J (2014) Rapid measurements of orientation and rotation in Ex-Vivo muscle possible by studying small number of cross-bridges. In “Fluorescence Microscopy: Super-resolution and Other Novel Techniques”, 35-48, A. Cornea and P.M. Conn eds. Academic Press, Amsterdam.

Valapala M, Maji S, Borejdo J, Vishwanatha JK (2014) Cell surface translocation of annexin A2 facilitates glutamate-induced extracellular proteolysis. J Biol. Chem. 2014 Apr 17.

Huang W, Liang J, Kazmierczak K, Muthu P, Duggal D, Farman GP, Sorensen L, Pozios I, Abraham TP, Moore JR, Borejdo J, Szczesna-Cordary D. (2014) Hypertrophic cardiomyopathy associated Lys104Glu mutation in the myosin regulatory light chain causes diastolic disturbance in mice. J Mol Cell Cardiol. 2014 Sep, 74:318-29.

Nagwekar J, Duggal D, Rich R, Raut S, Fudala R, Gryczynski I, Gryczynski Z, Borejdo J. The Spatial Distribution of Actin and Mechanical Cycle of Myosin Are Different in Right and Left Ventricles of Healthy Mouse Hearts. (2014) Biochemistry Dec 9;53(48):7641-9. doi: 10.1021/bi501175s.

Rice Q, Raut S, Chib R, Gryczynski Z, Gryczynski I, Zhang W, Zhong X, Abdel-Fattah M, Tabibi B, Seo J.Fractional Contributions of Defect-Originated Photoluminescence from CuInS2/ZnS Corshells for Hybrid White LEDs. (2014) Journal of Nanomaterials, Volume 2014, Article ID 979875, 7p.

Midde K, Rich R, Saxena A, Gryczynski I, Borejdo J, Das HK. Membrane Topology of Human Presenilin-1 in SK-N-SH Cells Determined by Fluorescence Correlation Spectroscopy and Fluorescent Energy Transfer. (2014) Cell Biochem. Biophys. 70: 923-932.

Raut S, Chib R, Butler S, Borejdo J, Gryczynski Z, Gryczynski I. Evidence of energy transfer from tryptophan to BSA/HAS protected gold nanoclusters. (2014) Methods Appl. Fluoresc. 2, 035004 (6pp).

Shumilov D, Rich RM, Gryczynski I, Raut S, Gryczynski K, Kimball J, Doan H, Sorensen T J, Laursen BW, Borejdo J, Gryczynski Z. Generating multiple-pulse bursts for enhanced fluorescence detection. (2014) Methods Appl Fluoresc. 2, 024009 (9pp).

Shumilov D, Popov A, Fudala R, Akopova I, Gryczynski I, Borejdo J, Gryczynski Z, Grygorczyk R. Real-time imaging of exocytotic mucin release and swelling in Calu-3 cells using acridine orange. (2014) Methods 66, 312-324.

Rich RM, Gryczynski I, Fudala R, Borejdo J, Stankowska DL, Krishnamoorthy RR, Raut S, Maliwal BP, Shumilov D, Doan H, Gryczynski Z. Multiple-pulse pumping for enhanced fluorescence detection and molecular imaging in tissue. (2014) Methods 66, 292-298.

Raut SL, Fudala R, Rich R, Kokate RA, Chib R, Gryczynski Z, Gryczynski I. Long lived BSA Au clusters as a time gated intensity imaging probe. (2014) Nanoscale 6, 2594-2597.

Chib R, Raut S, Sabnis S, Singhal P, Gryczynski Z, Gryczynski I. Associated anisotropy decays of ethidium bromide interacting with DNA. (2014) Methods. Appl. Fluores. 2, 015003 (6pp).

Kusba J, Grajek H, Gryczynski I. Secondary emission influenced fluorescence decay of a homogeneous fluorophore solution. (2014) Methods Appl. Fluoresc. 2, 015001 (11pp).

Raut S, Rich R, Fudala R, Butler S, Kokate R, Gryczynski Z, Luchowski R, Gryczynski I. Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores. (2013)Nanoscale 6, 385-391.

Fudala R, Rich R, Mukerjee A, Ranjan AP, Vishwanatha JK, Kurdowska A, Gryczynski Z, Borejdo J, Gryczynski I. Fluorescence Detection of MMP-9. II. Ratiometric FRET-Based Sensing With Dually Labeled Specific Peptide. (2013) Curr. Pharmaceut. Biotechn. 14, 1134-1138.

Raut SL, Rich R, Fudala R, Kokate R, Kimball JD, Borejdo J, Vishwanatha JK, Gryczynski Z, Gryczynski I.BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme. (2013) Curr. Pharm. Biotechn. 14, 1139-1144.

Brooks MM, Neelam S, Fudala R, Gryczynski I, Cammarata PR. Lenticular mitoprotection. Part I A: Monitoring mitochondrial depolarization with JC-1 and artifactual fluorescence by the glycogen synthase kinase-3beta inhibitor, SB216763. (2013) Molecular Vision, 19: 1406-1412.

Maliwal BP, Fudala R, Raut S, Kokate R, Sorensen TJ, Laursen BW, Gryczynski, Z, Gryczynski I. Long-Lived Bright Red Emitting Azaoxa-Triangulenium Fluorophores. (2013) PLOS ONE, 8 (5), e63043.

Chib R, Raut S, Fudala R, Chang A, Mummert M, Rich R, Gryczynski Z, Gryczynski I. FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer. (2013) Curr. Pharmac. Biotechn. 14, 470-474.

Sorensen TJ, Thyrhaug E, Szabelski M, Gryczynski I, Gryczynski Z, Laursen BW. AzadioxaTriAnulenium: a long fluorescence lifetime fluorophore for large biomolecule binding assay. (2013) Methods Appl. Fluoresc. 1, 025001(6pp).

Rich RM, Mummert M, Gryczynski Z, Borejdo J, Sorensen TJ, Laursen BW, Foldes-Papp Z, Gryczynski I, Fudala R. Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAnulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC). (2013) Anal. Bioanal. Chem. 405, 4887-4894.

Thyrhaug E, Sorensen TJ, Gryczynski I, Gryczynski Z, Laursen BW. Polarization and Symetry of Electronic Transitions in Long Fluorescence Lifetime Triangulenium Dyes. (2013) J. Phys. Chem. A 117, 2160-2168.

Midde K, Rich R, Marandos P, Fudala R, Li A, Gryczynski I, Borejdo J. Comparison of Orientation and Rotational Motion of Skeletal Muscle Cross-bridges Containing Phosphorylated and Dephosphorylated Myosin Regulatory Light Chain. (2013) J. Biol. Chem. 288 (10) 7012-7023.

Raut S, Chib R, Shumilov D, Gryczynski Z, Gryczynski I. Polarization properties of fluorescentBSA protected Au25 nanoclusters. (2013) Nanoscale 5, 3441-3446.

Raut SL, Shumilov D, Chib R, Rich R, Gryczynski Z, Gryczynski I. Two photon induced luminescence of BSA protected gold clusters. (2013) Chem. Phys. Lett. 561-562, 74-76.

Rich RM, Stankowska DL, Maliwal BP, Sorensen TJ, Laursen BW, Krishnamoorthy RR, Gryczynski Z, Borejdo J, Gryczynski I, Fudala R. Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore. (2013) Anal. Bioanal. Chem. 405, 2065-2075.

Krupa A, Fudala R, Florence JM, Tucker T, Allen TC, Standiford TJ, Luchowski R, Fol M, Rahman M, Gryczynski Z, Gryczynski I, Kurdowska AK. Bruton’s Tyrosine Kinase Mediates FcgammaRIIa/Toll-like Receptor-4 Receptor Crosstalk in Human Neutrophils. (2013) Am. J. Respir. Cell. Mol. Biol. 48, (2), 240-249.

Dongre P, Mathew S, Akopova I, Gryczynski I, Mathew P. YY1 and unique DNA repeat element regulates the transcription of mouse CS1 (CD319, SLAMF7) gene. (2013) Mol. Immunol. 54, 254-263.

Grobelna B, Synak A, Bojarski P, Szczodrowski K, Kuklinski B, Raut S, Gryczynski I. Synthesis and luminescence characteristics of Dy3+ ions in silica Xerogels doped with Ln2-xDyx(WO4)3. (2013) Optical Materials 35, 456-461.

Huang X, Gonzalez-Rodriguez R, Rich R, Gryczynski Z, Coffer JL Fabrication and size dependent properties of porous silicon nanotube arrays, Chemical Communications. 49 (2013) 5760–5762.

Borejdo J, Rich R, Midde K. Mesoscopic analysis of motion and conformation of cross-bridges, Biophysical Reviews. 4 (2012) 299–311.

Raut S, Rich R, Akopova I, Thyrhaug E, Shtoyko T, Shumilov D, Gryczynski Z, Gryczynski I. Fluorescent polyelectrolyte capped silver nanoclusters: Optimization and spectroscopic evaluation. (2012) Chem. Phys. Lett. 549, 72-76.

Komissarov AA, Stankowska D, Krupa A, Fudala R, Florova G, Florence J, Fol M, Allen TC, Idell S, Matthay MA, Kurdowska AK. Novel aspects of urokinase function in the injured lung: role of α2-macroglobulin. Am J Physiol Lung Cell Mol Physiol. (2012) Oct 12. 303:L1037-L1045. doi:10.1152/ajplung.00117.2012.

Rich RM, Mummert M, Foldes-Papp Z, Gryczynski Z, Borejdo J, Gryczynski I, Fudala R. Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy. J Photochem Photobiol B. 2012 Nov 5;116:7-12. doi: 10.1016/j.jphotobiol.2012.07.007.

Rich R, Li J, Fudala R, Gryczynski Z, Gryczynski I, Mandecki W. Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays. Anal Bioanal Chem. 2012 Nov;404(8):2223-31. doi: 10.1007/s00216-012-6369-9.

Dibas A, Jiang M, Fudala R, Gryczynski I, Gryczynski Z, Clark AF, Yorio T. Fluorescent protein-labeled glucocorticoid receptor alpha isoform trafficking in cultured human trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2012 May 17;53(6):2938-50.

Lewkowicz, A., Bojarski, P., Synak, A., Grobelna, B., Akopova, I., Gryczynski, I., Kulak, L.  Concentration-Dependent Fluorescence Properties of Rhodamine 6G in Titanium Dioxide and Silicon Dioxine Nanolayers. (2012) J. Phys. Chem. C 116, 12304-12311.

Thamake S, Raut S, Gryczynski Z, Ranjan A, Vishwanatha JK. Alendronate coated poly-lactic-co-glycolic acid (PLGA) nanoparticles for active targeting of metastatic breast cancer. Biomaterials 33 (2012) 7164-7173.

Borejdo J, Rich R, Midde K (2012) Mesoscopic Analysis of Skeletal Muscle Performance, In: Biophysical Reviews, J. Garnier, Ed, 4(4):299-31.

Huang W, Liang J, Kazmierczak K, Muthu P, Duggal D, Farman GP, Sorensen L, Pozios I, Abraham TP, Moore JR, Borejdo J, Szczesna-Cordary D. (2014) Hypertrophic cardiomyopathy associated Lys104Glu mutation in the myosin regulatory light chain causes diastolic disturbance in mice. J Mol Cell Cardiol. 2014 Sep, 74:318-29.

Seo, J., Fudala, R., Kim, W-J., Rich, R., Tabbi, B., Cho, H., Gryczynski, Z., Gryczynski, I., Yu, W.  Hybrid optical materials of plasmon-coupled CdSe/ZnS coreshells for photonic applications. (2012) Optical Materials Express 2 (8) 1026-1039.

Arabski M, Fudala R, Koza A, Wasik S, Futoma-Koloch B, Bugla-Ploskonska G, Kaca W.  The presence of anti-LPS antibodies and human serum activity against Proteus mirabilis S/R forms in correlation with TLR4 (Thr399Ile) gene polymorphism in rheumatoid arthritis. Clin Biochem. 2012 Jun 27. doi:10.1016/j.clinbiochem.2012.06.021

Fudala R, Rich R, Mukerjee A, Ranjan AP, Vishwanatha JK, Kurdowska AK, Gryczynski Z, Borejdo J, Gryczynski I.  Fluorescence Detection of MMP-9.II. Ratiometric FRET-Based Sensing With Dually Labeled Specific Peptide. Curr Pharm Biotechnol. 2012 Feb 20. BSP/CPB/E-Pub/003734; doi: 10.2174/1389212223391712010

Fudala R, Rout S, Maliwal BP, Zerda TW, Gryczynski I, Simanek E, Borejdo J, Rich R, Akopova I, Gryczynski Z.  FRET Enhanced Fluorescent Nanodiamonds. Curr Pharm Biotechnol. 2012 Mar 6. . BSP/CPB/E-Pub/003752

Dibas A, Jiang M, Clark A.F., Fudala R, Gryczynski I, Gryczynski Z, Yorio T. Characterization of trafficking of RFP-GRα and EGFP-GRβ steroid receptor translocation in a transformed human trabecular meshwork cell-line. IVOS (2012), Article in press. doi:10.1167/iovs.11-8331.

D’Auria, S., Apicella, E., Staiano, M., Di Giovanni, S., Ruggiero, G., Rossi, M., Sarkar, P., Luchowski, R., Gryczynski, I., Gryczynski, Z. Engineering resonance energy transfer for advanced immunoassays: The case of celiac disease. (2012) Anal. Biochem. 425, 13-17.

Akopova, I., Tatur, S., Grygorczyk, M., Luchowski, R., Gryczynski, I., Gryczynski, Z., Borejdo, J., Grygorczyk, R.Imaging exocytosis of ATP-containing vesicles with TIRF microscopy in lung epithelial A549 cells. (2012) Purinergic Signaling 8:59-70.

Folmar, M., Shtoyko, T., Fudala, R., Akopova, I., Gryczynski, Z., Raut, S., Gryczynski, I. Metal enhanced fluorescence of Me-ADOTA.Cl dye by silver triangular nanoprisms on a gold film. (2012) Chem. Phys. Lett. 531, 126-131.

Gruszecki, W.I., Zubik, M., Luchowski, R., Grudzinski, W., Gryczynski, Z., Gryczynski, I. Spectroscopy of Photosynthetic Pigment-Protein Complex LHCII. (2012) Acta Phys. Polon. A 121, 397-400.

Maliwal, B.P., Raut, S.,Fudala, R., D’Auria, S., Marzullo, V.M., Luini, A., Gryczynski, I., Gryczynski, Z.Extending Forster resonance energy transfer measurements beyond 100A using common organic  fluorophores: enhanced transfer in the presence of multiple acceptors. (2012) J. Biomed. Optics 17(1) 011006-1- 8.

Fudala R, Mummert ME, Gryczynski Z, Rich R, Borejdo J, Gryczynski I. Lifetime-based sensing of the hyaluronidase using fluorescein labeled hyaluronic acid. (2012) Journal of Photochemistry and Photobiology B: Biology 106, 69-73.

more… (before 2012)

Book Chapters

Borejdo, J., Szczesna-Cordary, D., Muthu, P., Metticolla, P., Luchowski, R., Gryczynski, Z., Gryczynski, I.  Single Molecule Detection Approach to Muscle Study: Kinetics of a Single Cross-Bridge During Contraction of Muscle. (2012) In Spectroscopic Methods of Analysis, Humana Press, W.M. Bujalowski (Ed.), pp. 311-334.

Gruszecki, W.I., Luchowski, R., Grudzinski, W., Gryczynski, Z., Gryczynski, I.   Photosynthetic Antenna Complex LHCII Studied with Novel Fluorescence Techniques. (2012) In Spectroscopic Methods of Analysis, Humana Press, W.M. Bujalowski (Ed.), pp. 263-269.

Gryczynski, I., Luchowski, R., Matveeva, E. G., Shtoyko, T., Sarkar, P., Borejdo, J., Akopova, I., Gryczynski, Z.  Meteal-Enhanced Immunoassays. (2012) In Spectroscopic Methods of Analysis, Humana Press, W.M. Bujalowski (Ed.), pp. 217-229.

Gruszecki, W.I., Luchowski, R., Wasko, P., Gryczynski, Z., Gryczynski, I. Molecular Organization of Polyene Antibiotic Amphotericin B Studied by Means of Fluorescence  Technique. (2012) In Spectroscopic Methods of Analysis, Humana Press, W.M. Bujalowski (Ed.), pp. 57-65.

Evgenia G. Matveeva, Ignacy Gryczynski, Zygmunt Gryczynski, Ewa M. Goldys
Fluorescence Applications in Biotechnology and the Life Sciences by Ewa M. Golgys (2009);
Chapter 16. Fluorescence immunochemical detection of analytes.

Ignacy Gryczynski, Rafal Luchowski, Shashank Bharill, Julian Borejdo, and Zygmunt Gryczynski
FLIM Microscopy in Biology and Medicine (Hardcover) by Ammasi Periasamy (Editor), Robert M. Clegg (Editor) (2009);
Chapter 12. Nonlinear Curve-Fitting Methods for Time-Resolved Data Analysis.

Gryczynski, Z., Matveeva, E.G., Calander, N., Zhang, J., Lakowicz, J.R., Gryczynski, I. (2007) Surface Plasmon Coupled Emission, pp. 247-265, in Surface Plasmon Nanophotonics (Brongersma, M.L., Kik, P.G., eds.) Springer, Dordrecht , The Netherlands .

Basics of Fluorescence and FRET (2005) in Molecular Imaging (A. Periasamy and R.N. Day, eds.) Oxford University Press, pp.21-56.