Lab protocols

Tutorial and method articles

Kejin Hu, I. Slukvin, (2013), Generation of transgene-free iPSC lines form Human normal and Neoplastic Blood cells using episomal vectors. Methods in Molecular Biology, Vol. 997, Chapter 13, pp 163-176. (PDF file for download, document size, 558 KB)
Z. Shao, R. Cevallos, M.E. Hossain, Kejin Hu*. (2021) Reprogramming of human fibroblasts to pluripotency by GFP-labeled factors in the chemical defined media, Nuclear Reprogramming – Methods in Molecular Biology. Chapter 7, vol. 2239:101-116. (PDF file for download, file size 380 KB)
R. Cevallos, M.E. Hossain, Kejin Hu*. (2021) Characterization of Human iPSCs by surface markers and signature pluripotency genes. Nuclear Reprogramming – Methods in Molecular Biology, Chapter 15, vol. 2239:235-250. (PDF file for download, file size, 1 MB)
Kejin Hu*. (2021) Become competent in one day in preparation of RNA-seq heat maps for novices without prior R experience. Nuclear Reprogramming – Methods in Molecular Biology, Chapter 17, vol. 2239:269-303. (PDF file for download, 4.6 MB)
Kejin Hu*. Become competent in one day in preparation of boxplots and violin plots for novices without prior R experience. Methods and Protocols. 3(4):64. (Link for download).

RNA-Seq Analyses Using HPC: A Tutorial for Bench Scientists, BookBaby ASIN‏: ‎ B0BHZVWFNL, October 11, 2022, (PDF file for download, 7 MB)