Light Microscopy Resources

Zeiss LSM 880 Confocal with AiryScan and AiryScan Fast Mode

This system is based off a fully motorized Axio Observer 7 Inverted Microscope with Motorized Scanning Stage with Piezo Z and includes the following:

LSM Detectors (Spectral detection of 4 channel with two GaAsP):

• 2 multi-anode and 1 GaAsP PMT
• Includes additional Airyscan GaAsP detector for super-resolution and Fast Mode
• Transmitted light detector T-PMT for detection of DIC, IR-DIC with laser illumination

Incubator Enclosure – Maintains user set CO2 level, temperature & humidity around the sample stage & objectives. For live cell experiments.

Installed Lasers:

• 405 nm Diode Laser 11.5 mW
• Argon Laser: 458/488/514 nm 15.4 mW
• 561 nm DPSS Laser 13 mW
• 633 nm HeNe Laser 2.3 mW

Objectives:

• 5x/0.16 EC Plan-Neofluar 5x/0.16 WD=18.5
• 10x/.30 EC Plan-Neofluar 10x/0.30 WD=5.2
• 20x/0.8 Objective Plan-Apochromat 20x/ 0.8 WD = 0.55
• 63x/1.4 Oil Plan-Apochromat 63x/1.40 Oil

Zeiss LSM 880 Confocal Upright Microscope with Multiphoton System

This system is based off a fully motorized Axio Observer 7 Upright  Microscope with Motorized Scanning Stage with Piezo Z capable of accommodating small animals and includes the following:

LSM Detectors (Spectral detection of 4 channel with two GaAsP):

• 2 channel green/red GaAsP NDD for MP
• Transmitted light detector T-PMT for detection of DIC, IR-DIC with laser illumination
• Spectral detection 3 ch with one GaAsP

Installed Lasers:

• Mai Tai HP Tunable Laser (690-1040 nm) Mai Tai HP provides more than 300 nm (690–1040 nm) in useable tuning range with over 2.5 W of average power and a pulse width of less than 100 fs
• 405 nm Diode Laser 11.5 mW
• Argon Laser: 458/488/514 nm 15.4 mW
• 561 nm DPSS Laser 13 mW
• 633 nm HeNe Laser 2.3 mW

Objectives:

• 5x/0.16 EC Plan-Neofluar 5x/0.16 WD=18.5
• 20x/1.0 Corr nd=1.38 (WD=5.6mm), Clr PApo

Zeiss LSM 510

Meta-head – for normal confocal including a spectrophotometric linear ccd array

Live-scan head – for capturing short duration events (i.e. Calcium Sparks). Uses line-scanning rather than point scanning (not as high a resolution image as with the Meta-head)

Incubator Enclosure – Maintains user set CO2 level, temperature & humidity around the sample stage & objectives. For live cell experiments.

Installed Lasers:

Diode 405nm -50mW
Argon/2 561nm
Diode 488nm -100mW
DPSS 532nm -75mW
DPSS 561nm -10mW
HeNe 633nm
Diode 635nm -35mW

Objectives:

10x, 0.45NA, 2 mm WD (air DIC), Plan-Apochromat
20x, 0.8NA, 0.55 mm WD (air DIC), Plan-Apochromat
63x, 1.4NA, 0.19 mm WD (oil DIC),Plan-Apochromat
40x, 1.2NA, 0.28 WD (water), C-Apochromat