Light Microscopy Resources

These are the resources the Microscopy Core Facility has for viewing samples by light microscopy

Zeiss LSM 880 Confocal with AiryScan and AiryScan Fast Mode

This system is based off a fully motorized Axio Observer 7 Inverted Microscope with Motorized Scanning Stage with Piezo Z and includes the following:

LSM Detectors (Spectral detection of 4 channel with two GaAsP)

  • 2 multi-anode and 1 GaAsP PMT
  • Includes additional Airyscan GaAsP detector for super-resolution and Fast Mode
  • Transmitted light detector T-PMT for detection of DIC, IR-DIC with laser illumination

Incubator Enclosure – Maintains user set CO2 level, temperature & humidity around the sample stage & objectives. For live cell experiments.

Installed Lasers

  • 405 nm Diode Laser 11.5 mW
  • Argon Laser: 458/488/514 nm 15.4 mW
  • 561 nm DPSS Laser 13 mW
  • 633 nm HeNe Laser 2.3 mW

Objectives

  • 5x/0.16 EC Plan-Neofluar 5x/0.16 WD=18.5
  • 10x/.30 EC Plan-Neofluar 10x/0.30 WD=5.2
  • 20x/0.8 Objective Plan-Apochromat 20x/ 0.8 WD = 0.55
  • 63x/1.4 Oil Plan-Apochromat 63x/1.40 Oil

Zeiss LSM 880 Confocal Upright Microscope with Multiphoton System

This system is based off a fully motorized Axio Observer 7 Upright  Microscope with Motorized Scanning Stage with Piezo Z capable of accommodating small animals and includes the following:

LSM Detectors (Spectral detection of 4 channel with two GaAsP)

  • 2 channel green/red GaAsP NDD for MP
  • Transmitted light detector T-PMT for detection of DIC, IR-DIC with laser illumination
  • Spectral detection 3 ch with one GaAsP

Installed Lasers

  • Mai Tai HP Tunable Laser (690-1040 nm) Mai Tai HP provides more than 300 nm (690–1040 nm) in useable tuning range with over 2.5 W of average power and a pulse width of less than 100 fs
  • 405 nm Diode Laser 11.5 mW
  • Argon Laser: 458/488/514 nm 15.4 mW
  • 561 nm DPSS Laser 13 mW
  • 633 nm HeNe Laser 2.3 mW

Objectives

  • 5x/0.16 EC Plan-Neofluar 5x/0.16 WD=18.5
  • 20x/1.0 Corr nd=1.38 (WD=5.6mm), Clr PApo

Zeiss LSM 510

Meta-head – for normal confocal including a spectrophotometric linear ccd array

Live-scan head – for capturing short duration events (i.e. Calcium Sparks). Uses line-scanning rather than point scanning (not as high a resolution image as with the Meta-head)

Incubator Enclosure – Maintains user set CO2 level, temperature & humidity around the sample stage & objectives. For live cell experiments.

Installed Lasers

  • Diode 405nm -50mW
  • Argon/2 561nm
  • Diode 488nm -100mW
  • DPSS 532nm -75mW
  • DPSS 561nm -10mW
  • HeNe 633nm
  • Diode 635nm -35mW

Objectives

  • 10x, 0.45NA, 2 mm WD (air DIC), Plan-Apochromat
  • 20x, 0.8NA, 0.55 mm WD (air DIC), Plan-Apochromat
  • 63x, 1.4NA, 0.19 mm WD (oil DIC),Plan-Apochromat
  • 40x, 1.2NA, 0.28 WD (water), C-Apochromat

This page was last modified on October 31, 2019