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Welcome to the official home of STRait Razor — the STR Allele Identification Tool! The current stable version of this program is v1.5. The complete v1.5 package can be found below, in .zip format. Updates to this program will be posted to this page as they are created.
STRait Razor v2.5 is now available for download. In addition to the improvements listed for v2.0, this latest version adds eight additional loci. As well as, a more robust detection system for potential microvariants. To
-updated locus config file
-added ‘allsequences.txt’ file for sequence-based analysis
-updated locus config file
-added support for each base (i.e., all microvariants) within the allelic range
-updated flanks for CSF1PO, D3S1358;
-added loci: D20S482; D6S1043; D9S1122; DXS10103; DXS10148; DYS387S1; D1S1677; D9S2157
-added locus.config file support for individual kits
-PGM 24 Plex
Excel-based Analysis Files*
Sequence-based Analysis v1.2
In order to utilize the potential sequence variation at each locus, the STRait Razor Analysis workbook was developed. This workbook allows users to take the STRait Razor output and perform more in depth analyses. Data may be exported into a separate sheet for fast compilation of a dataset. Two versions of this file are available**.
The standard edition*** is able to process up to 50k unique sequences and is more suitable for large multiplexes or samples with a high number of unique sequences (i.e., mixtures).
The lite edition is able to process 5k unique sequences and is suitable for small multiplexes or single source samples.
**Note: Choose your own adventure!!! When the lite edition of STRait Razor Analysis is
just not lite enough still too cumbersome for your dataset or the computational capabilities of your computer, apply the following changes to the workbook to reduce processing times.
***Note: The standard edition is now most suitable only for high read depth sequencing (e.g., PowerSeq runs with 100k X coverage). The LITE version is recommended for most applications.
-Added batch processing for 24 samples (read one only) or 12 samples (read one and two)
-Changed Sequence output to a single strand consistent with the locus.config file
-Added the Allele Export tab to give added flexibility
If simple length-based data are desired, STRait RazorGenotyper will convert raw STRait Razor data into a final genotype while also combining reads (if applicable). Data may be exported into a separate sheet for fast compilation of a dataset.
-Added tab ‘Advanced Read Combiner’; allows combination of reads 1 & 2 of mixed samples
STRait Razor Histogram Generator converts RazorGenotyper and Sequence Analysis output into mock electropherograms for easy data visualization.
*Please note these files contain macros that must be enabled for proper functionality. All workbooks are in constant development and feedback is not only desired but expected to improve these workbooks. Any help provided is greatly appreciated. Please address Excel comments to Jonathan.King@unthsc.edu