Laboratory Faculty & Staff

 September2017


Laboratory Faculty & Staff | R&D Lab Publications | Excel Workbooks | mitoSave

STRait Razor |Visiting Scientists | Notable Former Students |AutoCurE


bruce

Bruce Budowle, Professor

Dr. Bruce Budowle received a Ph.D. in Genetics in 1979 from Virginia Polytechnic Institute and State University. From 1979-1982, Dr. Budowle was a postdoctoral fellow at the University of Alabama at Birmingham. Working under a National Cancer Institute fellowship, he carried out research predominately on genetic risk factors for diseases such as insulin dependent diabetes mellitus, melanoma, and acute lymphocytic leukemia.From 1983-2009, Dr. Budowle worked at the FBI Laboratory Division to carry out research, development, and validation of methods for forensic biological analyses. Dr. Budowle has contributed to the fundamental sciences as they apply to forensics in analytical development, population genetics, statistical interpretation of evidence, and in quality assurance. Some of his technical efforts have been: 1) development of analytical assays for typing a myriad of protein genetic marker systems, 2) designing electrophoretic instrumentation, 3) developing molecular biology analytical systems to include RFLP typing of VNTR loci and PCR-based SNP assays, VNTR and STR assays, and direct sequencing methods for mitochondrial DNA, and 4) new technologies; and 5) designing image analysis systems. Dr. Budowle has worked on laying some of the foundations for the current statistical analyses in forensic biology and defining the parameters of relevant population groups. He has published more than 490 articles, made more than 580 presentations (many of which were as an invited speaker at national and international meetings), and testified in well over 250 criminal cases in the areas of molecular biology, population genetics, statistics, quality assurance, and forensic biology. In addition, he has authored or co-authored books on molecular biology techniques, electrophoresis, protein detection, and microbial forensics. Dr. Budowle has been directly involved in developing quality assurance (QA) standards for the forensic DNA field. He has been a chair and member of the Scientific Working Group on DNA Methods, Chair of the DNA Commission of the International Society of Forensic Genetics, and a member of the DNA Advisory Board. He was one of the original architects of the CODIS National DNA database, which maintains DNA profiles from convicted felons, from evidence in unsolved cases, and from missing persons.Dr. Budowle’s efforts over the past decade also have focused on counter terrorism specifically efforts involving microbial forensics and bioterrorism. Dr. Budowle was involved directly in the scientific aspects of the anthrax letters investigation and has been one of the architects of the field of microbial forensics. He has been the chair of the Scientific Working Group on Microbial Genetics and Forensics (hosted by the FBI), whose mission was to set QA guidelines, develop criteria for biologic and user databases, set criteria for a National Repository, and develop forensic genomic applications. He currently serves on other government working groups related to microbial forensics. He also has served on the Steering Committees or been a co-organizer for the Colloquia on Microbial Forensics sponsored by American Society of Microbiology, Microbial Forensics Meetings, hosted by DHS, held at The Banbury Center in the Cold Spring Harbor Laboratory, and a meeting on Microbial Evolution and Cutting Edge Tools for Outbreak Investigations, hosted by the Centers for Disease Control and Prevention. He has published a number of articles (see below) on microbial forensics on topics such as attribution, quality assurance, population genetics, next generation sequencing technology, and sample collection. His current efforts at UNTHSC continue to focus on the areas of human forensic identification, microbial forensics, and emerging infectious disease.


jonathan

 

Jonathan King, Laboratory Manager

Jonathan King is originally from North Carolina but has lived in Texas long enough to be considered a naturalized Texan. He received his MS from Tarleton State University in 2009 with a research focus in capturing novel polymorphic Indels from agricultural pathogens. He has been the laboratory manager for the research and development lab since March 2011. Jonathan’s current research projects include massively parallel sequencing, DNA repair, small amplicon markers, mitochondrial sequencing, novel DNA extraction techniques, and molecular medicine, just to name a few. When he is not working, Jonathan enjoys photography, gardening, and the culinary arts.

 


 

photo

Jennifer Churchill, Research Assistant Professor

Jennifer Churchill is currently a Research Assistant Professor at UNT Health Science Center’s Center for Human Identification. Jennifer received her Bachelor of Science degree in Biochemistry from Texas A&M University. Her undergraduate research at Texas A&M involved the application of molecular genetic technologies to the study of population and conservation genetics of the North American bison. Jennifer earned her Ph.D. in Biomedical Sciences specializing in Human and Molecular Genetics at the University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences. Her dissertation work focused predominantly on the use of linkage and next-generation sequencing technologies to identify novel autosomal dominant Retinitis Pigmentosa genes. Jennifer currently works with Dr. Bruce Budowle’s group at UNTHSC’s Center for Human Identification. Her research focuses on the development and application of human identification genetic marker analyses with massively parallel sequencing technologies including the validation and implementation of MPS for mitochondrial DNA analysis into UNTHSC Center for Human Identification’s Missing Persons Unit and Forensic Unit.

 


 

Angie_Ambers

 

 

Angie Ambers, Research Assistant Professor

Dr. Angie Ambers received her Ph.D. in Molecular Biology from the University of North Texas (UNT) with an emphasis in forensic genetics and human identification. Her dissertation involved an investigation of methods (e.g. whole genome amplification, DNA repair) for improving autosomal and Y-STR typing of degraded and low copy DNA from human skeletal remains and environmentally-damaged biological materials. Dr. Ambers also has master’s degrees in Forensic Genetics from the University of North Texas Health Science Center and in Criminology from the University of Texas at Arlington. Her thesis research involved developing and optimizing a DNA-based multiplex screening tool for the separation of fragmented and commingled skeletal remains. Since 2005, Dr. Ambers has been an adjunct professor at the University of North Texas (teaching Genetics, Heredity, and Human Anatomy andPhysiology). In 2008 she developed the curriculum for a course inForensic Molecular Biology, in which she teaches DNA analysis/methodology to undergraduate students enrolled in the FEPAC-accredited forensic science certificate program. Prior to pursuing her doctorate, Dr. Ambers was lead DNA analyst and lab manager of UNT’s DNA Sequencing Core Facility, and during that time had the opportunity to work on various ancient DNA projects involving archaeological specimens from Greenland. Her latest work has involved DNA testing of various historical human skeletal remains, including those of an American Civil War guerrilla scout, several Finnish World War II soldiers, and unidentified late-19th -century skeletal remains discovered in Deadwood, South Dakota. Dr. Ambers is currently a postdoctoral fellow at the IAG, specializing in characterization and identification of historical and archaeological human skeletal remains.

 


 

maiko

Maiko Takahashi, Post-doctoral Research Associate

Maiko was born and raised in Japan, and received her B.S.in pharmaceutical science and pharmacist license. Maiko worked for 10 years at the Criminal Investigation Laboratory of Tokyo Metropolitan Police Department as forensic scientist where she completed over 2,000 cases ofDNA typing for crime investigation. She earned her Ph.D.in Forensic Medicine from the University of Tokyo during the duty in TMPD. She now aims to develop novel methods that useful for forensic casework at UNTHSC. Her current research focus is the mixture DNA analysis using massively parallel sequencing technology. In her free time, Maiko enjoys boarding airplanes (just as a passenger), traveling and cooking.

 


 

Woerner

Augustus Woerner, Research Assistant Professor

August is a bioinformatics wiz. A true Perl in our eyes. If you R looking for someone to help you, he’s got better than a C+ rating on Yelp. In his spare time, he enjoys playing with his children, a hot cup of Java, and wrestling Pythons.

 

 


Magdalena

Magdalena Bus, Post-doctoral Research Associate

Magdalena received her Ph.D. in Poland. During her Ph.D. study, she worked with DNA collected non-invasively in the field and wild animals’ population genetic data. She is experienced in analyses of a limited quantity and severely degraded DNA from human and animal samples collected non-invasively in the field, from museum specimens, at crime scenes, or historical excavation sites. During her postdoctoral research at Uppsala University in Sweden, she was focused on the analysis of DNA from forensic and historical samples. The main objective of her research was to develop highly sensitive assays for analyses of challenging samples (e.g., human skeletal remains from a Swedish warship that has been in seawater for over 300 years, or ancient samples from excavations of Viking-age graves). Magdalena has substantial expertise in developing and optimizing sensitive techniques for low copy number DNA analyses such as Sanger sequencing, Pyrosequencing, and Massively Parallel Sequencing. Recently, she graduated from a postgraduate course Substantive and Procedural Criminal Law at Jagiellonian University. Currently, she is focused on the population genetics and massively parallel sequencing data analyses. She is interested in history and criminal law. Her free time she spends with her family and friends.


carey

 

Carey Davis, Ph.D. Candidate

Carey Davis is a PhD student at the University of North Texas Health Science Center. She completed her B.S. in biochemistry at the University of Virginia while working in the pathology department laboratory headed by Dr. Dennis Templeton. She then continued to Virginia Commonwealth University and received her M.S. in Forensic Science while working under the direction of Dr. Tracey Dawson Cruz on low copy number analysis. Since her arrival to UNTHSC, she has worked on many projects including: low copy number techniques, Y-STR development, and STR variant analysis. Her main research focuses on molecular autopsy and employing next generation sequencing techniques to the world of forensics. In her free time, she enjoys scuba diving, traveling, and working as a volunteer firefighter.

 


 Nicole Novroski

Nicole Novroski, Ph.D. Candidate

Nicole is a PhD Candidate under the guidance of Dr. Bruce Budowle at the University of North Texas Health Science Center.  She is native to Canada, where she completed her Bachelor’s Degree in Forensic Science and Biology at the University of Toronto.  She then worked with the Royal Canadian Mounted Police in Northern Alberta before moving on to the University at Albany, SUNY to complete her Master’s Degree in Forensic Molecular Biology.  Following graduation in 2011, Nicole spent some time at the NYC Office of Chief Medical Examiner Department of Forensic Biology as a Criminalist and left in 2013 to pursue her studies at UNTHSC.  Her current research focus is in STR mixture de-convolution using massively parallel sequencing.  When she isn’t studying, Nicole enjoys running, reading, volunteering, and spending quality time with her husband Lindsay and great dane Hank and Earl.

 


 

Rachelwiley

Rachel Wiley, Ph.D. Candidate

Rachel is a first year Ph.D. student currently rotating in Dr. Budowle’s laboratory at the University of North Texas Health Science Center. She grew up in Texas and received her Bachelors of Science in Biochemistry from the University of North Texas in May 2012. She continued her education in Washington, DC as she earned her Masters of Forensic Science in Forensic Molecular Biology at The George Washington University. While attending GWU, she interned with the Naval Criminal Investigative Services (NCIS) at the Washington DC Field Office serving as the Cold Case/Fraud/Forensic Intern. Her current research focuses on the development of a multiplex system sequencing the complete mitochondrial genome through the utilization of “mini” amplicons. When she is not hitting the books or spending time with family and friends, she likes watching sci-fi movies, reading, gaming, and dancing.

 

 

 


 

frankwendt

Frank Wendt, Ph.D. Candidate

Frank is a Biomedical Science Ph.D. Candidate under the mentorship of Dr. Bruce Budowle. He received his Bachelor of Science degree in Forensic Science, with a concentration in biology, from The Pennsylvania State University in 2013. His undergraduate research projects involved understanding how microorganisms thrive in, and contribute to, the sub-zero temperature ecosystems of Siberian, Antarctic, and Pennsylvania cold trap permafrost (Dr. Corien Bakermans), evaluation of rapid DNA technologies for field-forward applications (Dr. Mitchell Holland), and applications of massively parallel sequencing (MPS) to mitochondrial DNA, specifically for detecting transmission of heteroplasmy in maternal lineages (Dr. Mitchell Holland). Frank’s doctoral work in the Budowle Lab focuses primarily on in silico characterization and MPS interrogation of full-gene regions of opiate absorption, distribution, metabolism, and excretion (ADME) and analgesic response pharmacogenes. Genetic variations (e.g., single nucleotide polymorphisms, insertion/deletion polymorphisms, gene transversions, and/or copy number changes) in these genes produce phenotypic variation that may contribute to addiction, overdose, and even death and are an emerging concern for the personalized healthcare, forensic genetics, and pharmaceutical communities. The overall goal of his doctoral work is to develop and evaluate the utility of a comprehensive (full-gene) and combinatorial (multi-genic) metabolizer phenotype for molecular autopsy of deceased, tramadol-exposed Finns using genes encoding proteins responsible for various portions of the opiate ADME and response pathways. Frank also has been involved in collaborative side projects addressing topics of MPS and capillary electrophoresis assay development, Native American population genetics, design and implementation of MPS data analysis software, application of tandem mass spectrometry and statistical methods to screening for ignitable liquids in fire debris, and identification of opioid polypharmacy combinations of clinical and public health interests. Frank plans to defend his dissertation in Summer 2018.


sunny

Bing (Sunny) Song, Ph.D. Candidate

Sunny is a first year Ph.D. student doing a rotation in Dr. LaRue’s lab at the University of North Texas Health Science Center. She earned her bachelor degree of biological science at Fudan University, China. She used to study and work in Dr. Li Jin’s anthropology key laboratory, focusing on the research of ancestry information from chromosome haplo groups, more specifically haplo group N.Now she is researching on the identity of the Indel polymorphism in massively parallel sequence data and is going to design new panels for the human identification and ancestry information.

 


Allison2

Allison Sherier, Ph.D. Student

Allison Sherier is a PhD student at the University of North Texas Health Science center. She completed her M.S. in Animal Science Biotechnology at Oklahoma State University while working in the biotechnology laboratory headed by Dr. Jennifer Hernandez-Gifford. She then continued to Oklahoma State University Center for Health Sciences in Tulsa, OK where she received the National Science Foundation Bridge to the Doctorate Fellowship. She completed her M.S. in Forensic Science – Molecular Biology while working under the direction of Dr. Robert Allen on degradation of semen specific mRNA markers. When she is not studying, Allison enjoys photography, hiking, reading, volunteering with Cardigan Welsh Corgi National Rescue Trust, and spending time with her husband and dogs.

 


 

This page was last modified on April 10, 2018